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MTMR15 Recombinant antibody

MTMR15 Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240629E4

Cat no : 83622-6-RR

Print datasheet

Synonyms

hFAN1, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, FAN1, EC:3.1.21.-

验证数据展示

  • FC experiment of K-562 using 83622-6-RR

    FC experiment of K-562 using 83622-6-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4) (red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of A431 using 83622-6-RR

    FC experiment of A431 using 83622-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Mtmr15 Recombinant Antibody (83622-6-RR, Clone:240629E4)(red), or 0.25 ug Isotype Control (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83622-6-RR

    Affinity and Kinetic Characterization of 83622-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83622-6-RR against Human MTMR15 were performed. The affinity constant is 3.74 nM.

经过测试的应用

Positive FC (Intra) detected inK-562 cells, A431 cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83622-6-RR targets MTMR15 in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MTMR15 fusion protein Ag11651 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myotubularin related protein 15
Synonyms hFAN1, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, FAN1, EC:3.1.21.-
Calculated Molecular Weight 60 kDa, 114 kDa
GenBank Accession NumberBC047882
Gene Symbol MTMR15
Gene ID (NCBI) 22909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2M0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTMR15, also named as FAN1 and KIAA1018, is a nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. It is specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. MTMR15 has some isoforms with MW 114 kDa and 60 kDa. The MW will be migrated to 120-140 kDa and 63-68 kDa with modification.

实验方案

Product Specific Protocols
FC protocol for MTMR15 antibody 83622-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols