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验证数据展示

  • IHC staining of human tonsillitis using 21336-1-AP

    IHC staining of human tonsillitis using 21336-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21336-1-AP (MTMR3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 21336-1-AP

    IHC staining of human tonsillitis using 21336-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21336-1-AP (MTMR3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of SW 1990 using 21336-1-AP

    WB analysis of SW 1990 using 21336-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 21336-1-AP (MTMR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MTMR3 Polyclonal antibody

MTMR3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21336-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FYVE DSP1, KIAA0371, MTMR3, myotubularin related protein 3, ZFYVE10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSW 1990 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21336-1-AP targets MTMR3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 MTMR3 fusion protein Ag15514 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myotubularin related protein 3
别名 FYVE DSP1, KIAA0371, MTMR3, myotubularin related protein 3, ZFYVE10
计算分子量 1198 aa, 134 kDa
观测分子量 150 kDa
GenBank蛋白编号BC151217
基因名称 MTMR3
Gene ID (NCBI) 8897
RRIDAB_2878842
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13615
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTMR3 antibody 21336-1-APDownload protocol
IHC protocol for MTMR3 antibody 21336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent manner.

Authors - Shouji Zhang
View Article