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MTMR8 Polyclonal antibody

MTMR8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12299-1-AP

Print datasheet

Synonyms

Phosphatidylinositol-3-phosphate phosphatase, Phosphatidylinositol-3,5-bisphosphate 3-phosphatase, Myotubularin-related protein 8, myotubularin related protein 8, EC:3.1.3.95

验证数据展示

  • WB analysis of HeLa using 12299-1-AP

    WB analysis of HeLa using 12299-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 12299-1-AP

    IF Staining of A375 using 12299-1-AP

    Immunofluorescent analysis of A375 cells using 12299-1-AP (MTMR8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, A375 cells, human placenta tissue
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12299-1-AP targets MTMR8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MTMR8 fusion protein Ag2944 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myotubularin related protein 8
Synonyms Phosphatidylinositol-3-phosphate phosphatase, Phosphatidylinositol-3,5-bisphosphate 3-phosphatase, Myotubularin-related protein 8, myotubularin related protein 8, EC:3.1.3.95
Calculated Molecular Weight 704 aa, 79 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC012399
Gene Symbol MTMR8
Gene ID (NCBI) 55613
RRIDAB_2298075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTMR8 antibody 12299-1-APDownload protocol
IF protocol for MTMR8 antibody 12299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols