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验证数据展示

  • WB analysis using 83694-4-RR

    WB analysis using 83694-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83694-4-RR (MTMR9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83694-4-RR

    IF Staining of HeLa using 83694-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR9 antibody (83694-4-RR, Clone: 240167G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83694-4-RR

    FC experiment of HeLa using 83694-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83694-4-RR

    Affinity and Kinetic Characterization of 83694-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83694-4-RR against Human MTMR9 were performed. The affinity constant is below 1 pM.

MTMR9 Recombinant antibody

MTMR9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83694-4-RR

产品说明书

CloneNo. 240167G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

myotubularin related protein 9, MTMR8, LIP STYX, Inactive phosphatidylinositol 3-phosphatase 9, C8orf9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, U2OS cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, A549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83694-4-RR targets MTMR9 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MTMR9 fusion protein Ag34134 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myotubularin related protein 9
别名 myotubularin related protein 9, MTMR8, LIP STYX, Inactive phosphatidylinositol 3-phosphatase 9, C8orf9
计算分子量 63 kDa
观测分子量~60 kDa
GenBank蛋白编号BC022003
基因名称 MTMR9
Gene ID (NCBI) 66036
RRIDAB_3671297
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96QG7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).

实验方案

Product Specific Protocols
WB protocol for MTMR9 antibody 83694-4-RRDownload protocol
IF protocol for MTMR9 antibody 83694-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols