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MTPN Polyclonal antibody

MTPN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13508-1-AP

Print datasheet

Synonyms

V 1, Protein V-1, Protein V 1, GCDP

验证数据展示

  • WB analysis using 13508-1-AP

    WB analysis using 13508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human normal colon using 13508-1-AP

    IHC staining of human normal colon using 13508-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13508-1-AP (MTPN antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTPN antibody included
  • IF Staining of HepG2 using 13508-1-AP

    IF Staining of HepG2 using 13508-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTPN antibody (13508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, human brain tissue, HeLa cells, Jurkat cells
Positive IHC detected inhuman normal colon, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MTPN Ready-To-Use IHC Kit. MTPN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

13508-1-AP targets MTPN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MTPN fusion protein Ag4419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myotrophin
Synonyms V 1, Protein V-1, Protein V 1, GCDP
Calculated Molecular Weight 118 aa, 13 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC028093
Gene Symbol MTPN
Gene ID (NCBI) 136319
RRIDAB_2148129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58546
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myotrophin (MTPN) is a ubiquitously expressed 12 kDa cytoplamic protein that was first isolated from the hearts of spontaneously hypertensive rats. Protein sequence analysis revealed that MTPN was composed of 118 amino acids and was occupied by two and a half internal 33 amino acid ankyrin repeats. MTPN stimulates protein synthesis and increases the expression of a number of cardiac marker genes (such as atrial natriuretic factor and b-myosin heavy chain) in cardiomyocytes leading to hypertrophy. In skeletal muscle, MTPN is also a positive growth factor in promoting skeletal muscle growth.

实验方案

Product Specific Protocols
WB protocol for MTPN antibody 13508-1-APDownload protocol
IHC protocol for MTPN antibody 13508-1-APDownload protocol
IF protocol for MTPN antibody 13508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Obstet Gynecol

Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.

Authors - Roni Levy
View Article
humanIHC

J Clin Biochem Nutr

Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction.

Authors - Masataka Nomoto
View Article