MTR Polyclonal antibody
MTR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, mouse pancreas tissue |
Positive IHC detected in | human pancreas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A375 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25896-1-AP targets MTR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | MTR fusion protein Ag23156 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | 5-methyltetrahydrofolate-homocysteine methyltransferase |
Synonyms | cblG, Methionine synthase, MS, MTR |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | BC130616 |
Gene Symbol | MTR |
Gene ID (NCBI) | 4548 |
RRID | AB_2880287 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q99707 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for MTR antibody 25896-1-AP | Download protocol |
IHC protocol for MTR antibody 25896-1-AP | Download protocol |
IF protocol for MTR antibody 25896-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation. | ||
Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis. | ||
J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. | ||
Clin Epigenetics A transgenic mice model of retinopathy of cblG-type inherited disorder of one-carbon metabolism highlights epigenome-wide alterations related to cone photoreceptor cells development and retinal metabolism | ||
Nutrients Mild Choline Deficiency and MTHFD1 Synthetase Deficiency Interact to Increase Incidence of Developmental Delays and Defects in Mice. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. |