• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MTRF1 Polyclonal antibody

MTRF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11581-1-AP

Print datasheet

Synonyms

MRF 1, MRF1, MtRF 1, MTRF1, MTTRF1, RF1

验证数据展示

  • WB analysis of mouse liver using 11581-1-AP

    WB analysis of mouse liver using 11581-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11581-1-AP (MTRF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 11581-1-AP

    IHC staining of human pancreas using 11581-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 11581-1-AP (MTRF1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman pancreas tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11581-1-AP targets MTRF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MTRF1 fusion protein Ag2170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial translational release factor 1
Synonyms MRF 1, MRF1, MtRF 1, MTRF1, MTTRF1, RF1
Calculated Molecular Weight 445 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC027731
Gene Symbol MTRF1
Gene ID (NCBI) 9617
RRIDAB_10734595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75570
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTRF1 antibody 11581-1-APDownload protocol
IHC protocol for MTRF1 antibody 11581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols