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验证数据展示

  • IP experiment of Jurkat using 27832-1-AP

    IP experiment of Jurkat using 27832-1-AP

    IP result of anti-MTSS1L (IP:27832-1-AP, 4ug; Detection:27832-1-AP 1:300) with Jurkat cells lysate 4000 ug.

  • WB analysis using 27832-1-AP

    WB analysis using 27832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27832-1-AP (MTSS1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27832-1-AP

    IHC staining of mouse brain using 27832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 27832-1-AP

    IF Staining of MCF-7 using 27832-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1L antibody (27832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 27832-1-AP

    IF Staining of MCF-7 using 27832-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27832-1-AP (MTSS1L antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MTSS1L Polyclonal antibody

MTSS1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 27832-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Protein MTSS 2, MTSS2, MTSS1-like protein, Actin-bundling with BAIAP2 homology protein 1, ABBA-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27832-1-AP targets MTSS1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MTSS1L fusion protein Ag27216 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 metastasis suppressor 1-like
别名 Protein MTSS 2, MTSS2, MTSS1-like protein, Actin-bundling with BAIAP2 homology protein 1, ABBA-1
观测分子量 68-70 kDa
GenBank蛋白编号BC002770
基因名称 MTSS1L
Gene ID (NCBI) 92154
RRIDAB_2880986
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ765P7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTSS2, also known as MTSS1L, contains a conserved N-terminal domain, called an IRSP53 /MIM homology domain (IMD) or inverse BAR domain, that is involved in plasma membrane dynamics (PMID: 20875796). MTSS2 potentiated PDGF-mediated formation of membrane ruffles and lamellipodia in fibroblasts, acting via RAC1 activation (PMID:14752106). MTSS2 is implicated in intellectual developmental disorders with ocular anomalies and distinctive facial features(PMID: 36067766).

实验方案

Product Specific Protocols
WB protocol for MTSS1L antibody 27832-1-APDownload protocol
IHC protocol for MTSS1L antibody 27832-1-APDownload protocol
IF protocol for MTSS1L antibody 27832-1-APDownload protocol
IP protocol for MTSS1L antibody 27832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

MicroRNA-23a promotes colorectal cancer cell migration and proliferation by targeting at MARK1.

Authors - Xiaoli Tang
View Article