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MUC1/CA15-3 C-terminal Recombinant antibody

MUC1/CA15-3 C-terminal Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240190G10

Cat no : 83311-4-RR

Print datasheet

Synonyms

CA15-3, CA153, MUC1, CA15 3, CA 15-3

验证数据展示

  • WB analysis using 83311-4-RR

    WB analysis using 83311-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83311-4-RR (MUC1/CA15-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83311-4-RR

    IHC staining of human stomach cancer using 83311-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with MUC1 antibody included
  • IHC staining of human tonsillitis using 83311-4-RR

    IHC staining of human tonsillitis using 83311-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC1 antibody included
  • IHC staining of human ovary cancer using 83311-4-RR

    IHC staining of human ovary cancer using 83311-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC1 antibody included
  • Affinity and Kinetic Characterization of 83311-4-RR

    Affinity and Kinetic Characterization of 83311-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83311-4-RR against Human CA15-3/MUC1 were performed. The affinity constant is 63.7 pM.

经过测试的应用

Positive WB detected inBxPC-3 cells, mouse lung tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MUC1 Ready-To-Use IHC Kit. MUC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83311-4-RR targets MUC1/CA15-3 C-terminal in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MUC1/CA15-3 C-terminal fusion protein Ag20366 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mucin 1, cell surface associated
Synonyms CA15-3, CA153, MUC1, CA15 3, CA 15-3
Calculated Molecular Weight 1264 aa, 123 kDa
Observed Molecular Weight 23-30 kDa
GenBank Accession NumberBC120975
Gene Symbol MUC1/CA15-3
Gene ID (NCBI) 4582
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.

实验方案

Product Specific Protocols
WB protocol for MUC1/CA15-3 C-terminal antibody 83311-4-RRDownload protocol
IHC protocol for MUC1/CA15-3 C-terminal antibody 83311-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols