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Species Reactivity
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验证数据展示

  • IHC staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

    IHC staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MUC2 antibody included
  • IHC staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

    IHC staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MUC2 antibody included
  • IF Staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

    IF Staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse colon using 27675-1-AP (same clone as 27675-1-PBS)

    IF Staining of mouse colon using 27675-1-AP (same clone as 27675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

  • IF Staining of rat small intestine using 27675-1-AP (same clone as 27675-1-PBS)

    IF Staining of rat small intestine using 27675-1-AP (same clone as 27675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 27675-1-AP (same clone as 27675-1-PBS)

    IF Staining of HepG2 using 27675-1-AP (same clone as 27675-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MUC2 antibody (27675-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 27675-1-AP (same clone as 27675-1-PBS)

    FC experiment of MCF-7 using 27675-1-AP (same clone as 27675-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.13 ug MUC2 Polyclonal antibody (27675-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

MUC2 Polyclonal antibody, PBS Only

MUC2 Polyclonal Antibody for IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 27675-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

MUC 2, MUC-2, Mucin 2, SMUC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MUC2 Ready-To-Use IHC Kit. MUC2 primary antibody included.

产品信息

27675-1-PBS targets MUC2 in IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MUC2 fusion protein Ag25800 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mucin 2, oligomeric mucus/gel-forming
别名 MUC 2, MUC-2, Mucin 2, SMUC
计算分子量 540 kDa
GenBank蛋白编号M94132
基因名称 MUC2
Gene ID (NCBI) 4583
RRIDAB_2880943
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.