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验证数据展示

  • WB analysis using 30457-1-AP

    WB analysis using 30457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30457-1-AP (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 30457-1-AP

    IF Staining of HepG2 using 30457-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (30457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MUC20 Polyclonal antibody

MUC20 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30457-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IF/ICC, ELISA

别名

FLJ14408, FLJ53153, KIAA1359, MUC 20, MUC20, Mucin 20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30457-1-AP targets MUC20 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 MUC20 fusion protein Ag31867 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mucin 20, cell surface associated
别名 FLJ14408, FLJ53153, KIAA1359, MUC 20, MUC20, Mucin 20
计算分子量 55 kDa, 71 kDa
观测分子量 70 kDa
GenBank蛋白编号BC029267
基因名称 MUC20
Gene ID (NCBI) 200958
RRIDAB_3086323
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N307
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

实验方案

Product Specific Protocols
WB protocol for MUC20 antibody 30457-1-APDownload protocol
IF protocol for MUC20 antibody 30457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols