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验证数据展示

  • WB analysis using 68714-1-Ig

    WB analysis using 68714-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68714-1-Ig (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68714-1-Ig

    IF Staining of HepG2 using 68714-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (68714-1-Ig, Clone: 1G9B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

MUC20 Monoclonal antibody

MUC20 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68714-1-Ig

产品说明书

CloneNo. 1G9B2

宿主/亚型

Mouse / IgG3

种属反应性

human, canine

应用

WB, IF/ICC, ELISA

别名

UNQ2782/PRO7170, Mucin-20, MUC-20, MUC 20, KIAA1359

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDCK cells, HEK-293 cells, HK-2 cells, A549 cells, SK-BR-3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68714-1-Ig targets MUC20 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, canine
免疫原 MUC20 fusion protein Ag31840 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 mucin 20, cell surface associated
别名 UNQ2782/PRO7170, Mucin-20, MUC-20, MUC 20, KIAA1359
计算分子量 55 kDa, 71 kDa
观测分子量 70 kDa
GenBank蛋白编号BC029267
基因名称 MUC20
Gene ID (NCBI) 200958
RRIDAB_3670407
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N307
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

实验方案

Product Specific Protocols
WB protocol for MUC20 antibody 68714-1-IgDownload protocol
IF protocol for MUC20 antibody 68714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols