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验证数据展示

  • IHC staining of human stomach using 20725-1-AP

    IHC staining of human stomach using 20725-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20725-1-AP (MUC5AC antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human stomach using 20725-1-AP

    IHC staining of human stomach using 20725-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20725-1-AP (MUC5AC antibody) at dilution of 1:200 (under 10x lens).

MUC5AC Polyclonal antibody

MUC5AC Polyclonal Antibody for IHC, ELISA
Cat No. 20725-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA and More (1)

Synonyms

MUC5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman stomach tissue, mouse stomach tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20725-1-AP targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 5AC, oligomeric mucus/gel-forming
Synonyms MUC5
Calculated Molecular Weight 527 kDa
GenBank Accession NumberNM_017511
Gene Symbol MUC5AC
Gene ID (NCBI) 4586
RRIDAB_3669342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC5AC is a member of the mucin family, a heterogeneous group of 21 abundant, high molecular weight O-glycoproteins that can be either secreted or membrane bound. Multiple cysteine-rich domains in both N- and C-terminal regions of MUC5AC are responsible for its disulfide-mediated polymerization, which is critical for gel-forming properties. (PMID: 26751774, PMID: 27845339)

实验方案

Product Specific Protocols
IHC protocol for MUC5AC antibody 20725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Mol Immunol

Knockdown of Brg1 reduced mucus secretion in HDM stimulated airway inflammation

Authors - Maozhu Xu
View Article