验证数据展示
经过测试的应用
Positive IHC detected in | human stomach tissue, mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
20725-1-AP targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | mucin 5AC, oligomeric mucus/gel-forming |
别名 | MUC5 |
计算分子量 | 527 kDa |
GenBank蛋白编号 | NM_017511 |
基因名称 | MUC5AC |
Gene ID (NCBI) | 4586 |
RRID | AB_3669342 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MUC5AC is a member of the mucin family, a heterogeneous group of 21 abundant, high molecular weight O-glycoproteins that can be either secreted or membrane bound. Multiple cysteine-rich domains in both N- and C-terminal regions of MUC5AC are responsible for its disulfide-mediated polymerization, which is critical for gel-forming properties. (PMID: 26751774, PMID: 27845339)
实验方案
Product Specific Protocols | |
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IHC protocol for MUC5AC antibody 20725-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |