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MUC5AC Polyclonal antibody

MUC5AC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30408-1-AP

Print datasheet

Synonyms

MUC5

验证数据展示

  • WB analysis using 30408-1-AP

    WB analysis using 30408-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30408-1-AP (MUC5AC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 30408-1-AP

    IHC staining of human stomach using 30408-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30408-1-AP targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MUC5AC fusion protein Ag30190 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 5AC, oligomeric mucus/gel-forming
Synonyms MUC5
Calculated Molecular Weight 586 kDa
Observed Molecular Weight290 kDa
GenBank Accession NumberNM_001304359
Gene Symbol MUC5AC
Gene ID (NCBI) 4586
RRIDAB_3669718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98088
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC5AC (Mucin-5AC ) is a gel-forming glycoprotein of the gastric and respiratory epithelium. It can protect mucous membranes from infection and chemical damage by binding to inhaled microorganisms and particles, which are then cleared by the mucociliary system. (PubMed:14535999,PubMed:14718370)

实验方案

Product Specific Protocols
WB protocol for MUC5AC antibody 30408-1-APDownload protocol
IHC protocol for MUC5AC antibody 30408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols