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验证数据展示

  • WB analysis using 30408-1-AP

    WB analysis using 30408-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30408-1-AP (MUC5AC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 30408-1-AP

    IHC staining of human stomach using 30408-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 30408-1-AP (MUC5AC antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MUC5AC Polyclonal antibody

MUC5AC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30408-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MUC5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30408-1-AP targets MUC5AC in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MUC5AC fusion protein Ag30190 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mucin 5AC, oligomeric mucus/gel-forming
别名 MUC5
计算分子量 586 kDa
观测分子量290 kDa
GenBank蛋白编号NM_001304359
基因名称 MUC5AC
Gene ID (NCBI) 4586
RRIDAB_3669718
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP98088
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC5AC (Mucin-5AC ) is a gel-forming glycoprotein of the gastric and respiratory epithelium. It can protect mucous membranes from infection and chemical damage by binding to inhaled microorganisms and particles, which are then cleared by the mucociliary system. (PubMed:14535999,PubMed:14718370)

实验方案

Product Specific Protocols
WB protocol for MUC5AC antibody 30408-1-APDownload protocol
IHC protocol for MUC5AC antibody 30408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols