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MUC8 Polyclonal antibody

MUC8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55489-1-AP

Print datasheet

Synonyms

MUC8, mucin 8

验证数据展示

  • WB analysis of A549 using 55489-1-AP

    WB analysis of A549 using 55489-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 55489-1-AP

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 55489-1-AP

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 55489-1-AP

    IF Staining of A549 using 55489-1-AP

    Immunofluorescent analysis of A549 cells using 55489-1-AP (MUC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55489-1-AP targets MUC8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 8
Synonyms MUC8, mucin 8
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33-38 kDa
GenBank Accession NumberU14383
Gene Symbol MUC8
Gene ID (NCBI) 4590
RRIDAB_11182275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mucin glycoproteins (Mucins) are major constituents of the glycocalyx that covers mucosal epithelium. MUC8 is one of the major mucins in the ethmoid mucosa and is upregulated by chronic inflammation. TNFa, IL-1b and a combination of both can significantly increase Mucin 8 mRNA levels, suggesting that a mixture of inflammatory mediators can synergistically increase secretion of mucin in human nasal epithelium. This antibody is specific to MUC8.

实验方案

Product Specific Protocols
WB protocol for MUC8 antibody 55489-1-APDownload protocol
IHC protocol for MUC8 antibody 55489-1-APDownload protocol
IF protocol for MUC8 antibody 55489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Diagnostics (Basel)

The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?

Authors - Martin Schicht
View Article