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MUM1 Polyclonal antibody

MUM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12682-1-AP

Print datasheet

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere, PWWP3A

验证数据展示

  • WB analysis using 12682-1-AP

    WB analysis using 12682-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12682-1-AP (MUM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 12682-1-AP

    IP experiment of Jurkat using 12682-1-AP

    IP result of anti-MUM1 (IP:12682-1-AP, 4ug; Detection:12682-1-AP 1:300) with Jurkat cells lysate 4000ug.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HT-1080 cells
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12682-1-AP targets MUM1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen MUM1 fusion protein Ag3371 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma associated antigen (mutated) 1
Synonyms EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere, PWWP3A
Calculated Molecular Weight 18 kDa, 79 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC008098
Gene Symbol MUM1
Gene ID (NCBI) 84939
RRIDAB_10642694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2TAK8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

实验方案

Product Specific Protocols
WB protocol for MUM1 antibody 12682-1-APDownload protocol
IP protocol for MUM1 antibody 12682-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Post-translational control of beige fat biogenesis by PRDM16 stabilization

Authors - Qiang Wang
View Article