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MUM1 Monoclonal antibody

MUM1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3B1D2

Cat no : 60353-1-Ig

Print datasheet

Synonyms

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere

验证数据展示

  • IF Staining of HeLa using 60353-1-Ig

    IF Staining of HeLa using 60353-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MUM1 antibody (60353-1-Ig, Clone: 3B1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of HEK-293 using 60353-1-Ig

    WB analysis of HEK-293 using 60353-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, A375 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells, A375 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

60353-1-Ig targets MUM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen MUM1 fusion protein Ag3371 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name melanoma associated antigen (mutated) 1
Synonyms EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere
Calculated Molecular Weight 18 kDa, 79 kDa
Observed Molecular Weight 78-80 kDa
GenBank Accession NumberBC008098
Gene Symbol MUM1
Gene ID (NCBI) 84939
RRIDAB_2881462
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ2TAK8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

实验方案

Product Specific Protocols
WB protocol for MUM1 antibody 60353-1-IgDownload protocol
IF protocol for MUM1 antibody 60353-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Clinical Impact of the Histopathological Index and Neuroimaging Features Status in Primary Central Nervous System Diffuse Large B-Cell Lymphoma: A Single-Center Retrospective Analysis of 51 Cases.

Authors - Zhou Qi
View Article