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验证数据展示

  • IHC staining of human kidney using 25619-1-AP

    IHC staining of human kidney using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 10x lens).

  • IHC staining of human kidney using 25619-1-AP

    IHC staining of human kidney using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 40x lens).

  • WB analysis of HEK-293 using 25619-1-AP

    WB analysis of HEK-293 using 25619-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25619-1-AP (MUPCDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MUPCDH Polyclonal antibody

MUPCDH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25619-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

CDHR5, MU PCDH, Mu protocadherin, MUCDHL, mucin like protocadherin, MUPCDH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25619-1-AP targets MUPCDH in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 MUPCDH fusion protein Ag22537 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mucin-like protocadherin
别名 CDHR5, MU PCDH, Mu protocadherin, MUCDHL, mucin like protocadherin, MUPCDH
计算分子量 845 aa, 88 kDa
观测分子量 70 kDa
GenBank蛋白编号BC038405
基因名称 MUPCDH
Gene ID (NCBI) 53841
RRIDAB_2880164
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUPCDH (Mu-protocadherin), also known as MUCDHL (Mucin and cadherin-like protein) and CDHR5 (Cadherin-related family member 5), is a member of the cadherin superfamily. It is involved in calcium-dependent cell adhesion and may have additional functions in terminally differentiated epithelia. The MUPCDH gene maps to 11p15.5, with highest expression in kidney, liver, colon and small intestine. Alternatively spliced transcript variants encoding different isoforms have been described. (PMID: 12167596; 21839066)

实验方案

Product Specific Protocols
WB protocol for MUPCDH antibody 25619-1-APDownload protocol
IHC protocol for MUPCDH antibody 25619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
View Article