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验证数据展示

  • IHC staining of human kidney using 25619-1-AP

    IHC staining of human kidney using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 10x lens).

  • IHC staining of human kidney using 25619-1-AP

    IHC staining of human kidney using 25619-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 40x lens).

  • WB analysis of HEK-293 using 25619-1-AP

    WB analysis of HEK-293 using 25619-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25619-1-AP (MUPCDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MUPCDH Polyclonal antibody

MUPCDH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25619-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

CDHR5, MU PCDH, Mu protocadherin, MUCDHL, mucin like protocadherin, MUPCDH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25619-1-AP targets MUPCDH in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen MUPCDH fusion protein Ag22537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin-like protocadherin
Synonyms CDHR5, MU PCDH, Mu protocadherin, MUCDHL, mucin like protocadherin, MUPCDH
Calculated Molecular Weight 845 aa, 88 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC038405
Gene Symbol MUPCDH
Gene ID (NCBI) 53841
RRIDAB_2880164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUPCDH (Mu-protocadherin), also known as MUCDHL (Mucin and cadherin-like protein) and CDHR5 (Cadherin-related family member 5), is a member of the cadherin superfamily. It is involved in calcium-dependent cell adhesion and may have additional functions in terminally differentiated epithelia. The MUPCDH gene maps to 11p15.5, with highest expression in kidney, liver, colon and small intestine. Alternatively spliced transcript variants encoding different isoforms have been described. (PMID: 12167596; 21839066)

实验方案

Product Specific Protocols
WB protocol for MUPCDH antibody 25619-1-APDownload protocol
IHC protocol for MUPCDH antibody 25619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
View Article