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MURC Polyclonal antibody

MURC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55464-1-AP

Print datasheet

Synonyms

cavin‐4, MURC

验证数据展示

  • WB analysis of mouse heart using 55464-1-AP

    WB analysis of mouse heart using 55464-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55464-1-AP (MURC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 55464-1-AP

    IHC staining of mouse heart using 55464-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55464-1-AP (MURC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55464-1-AP targets MURC in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name muscle-related coiled-coil protein
Synonyms cavin‐4, MURC
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 48-53 kDa
GenBank Accession NumberNM_001018116
Gene Symbol MURC
Gene ID (NCBI) 347273
RRIDAB_2881343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5BKX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MURC antibody 55464-1-APDownload protocol
IHC protocol for MURC antibody 55464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nutrients

Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection.

Authors - Jake S Russell
View Article