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MUS81 Polyclonal antibody

MUS81 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11018-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

EC:3.1.22.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11018-1-AP targets MUS81 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MUS81 fusion protein Ag1476 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MUS81 endonuclease homolog (S. cerevisiae)
别名 EC:3.1.22.-
计算分子量 61 kDa
观测分子量 52-72 kDa
GenBank蛋白编号BC009999
基因名称 MUS81
Gene ID (NCBI) 80198
RRIDAB_2147130
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NY9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).

实验方案

Product Specific Protocols
WB protocol for MUS81 antibody 11018-1-APDownload protocol
IF protocol for MUS81 antibody 11018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell

EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.

Authors - Antonio Marzio
  • KD Validated
WB

Elife

53BP1 and BRCA1 control pathway choice for stalled replication restart.

Authors - Yixi Xu
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