验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
11018-1-AP targets MUS81 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | MUS81 fusion protein Ag1476 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | MUS81 endonuclease homolog (S. cerevisiae) |
| Synonyms | EC:3.1.22.- |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 52-72 kDa |
| GenBank Accession Number | BC009999 |
| Gene Symbol | MUS81 |
| Gene ID (NCBI) | 80198 |
| RRID | AB_2147130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NY9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MUS81 antibody 11018-1-AP | Download protocol |
| IF protocol for MUS81 antibody 11018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.
| ||
