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MUTYH Polyclonal antibody

MUTYH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15237-1-AP

Print datasheet

Synonyms

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

验证数据展示

  • WB analysis of K-562 using 15237-1-AP

    WB analysis of K-562 using 15237-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15237-1-AP (MUTYH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15237-1-AP targets MUTYH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MUTYH fusion protein Ag7359 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mutY homolog (E. coli)
Synonyms hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 53 kDa, 65 kDa
GenBank Accession NumberBC003178
Gene Symbol MUTYH
Gene ID (NCBI) 4595
RRIDAB_2147408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

实验方案

Product Specific Protocols
WB protocol for MUTYH antibody 15237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols