验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, IFN beta treated THP-1 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
13278-1-AP targets MX2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | MX2 fusion protein Ag4020 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myxovirus (influenza virus) resistance 2 (mouse) |
| 别名 | p78-related protein, p78 related protein, Myxovirus resistance protein 2, MXB, Interferon-regulated resistance GTP-binding protein MxB |
| 计算分子量 | 715 aa, 82 kDa |
| 观测分子量 | 70-82 kDa |
| GenBank蛋白编号 | BC035293 |
| 基因名称 | MX2 |
| Gene ID (NCBI) | 4600 |
| RRID | AB_3669157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20592 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myxovirus resistance 2 (MX2, also known as MxB) is a member of both the dynamin family and the family of large GTPases. MX2 is an interferon-stimulated gene that inhibits the nuclear import of HIV-1 and interacts with the viral capsid and cellular nuclear transport machinery. MX2 mediates the effects of IFN signaling in a cell type and context-dependent manner that can influence cells sensitivity to MAPK pathway targeted therapies. MX2 is also associated with the tumorigenesis process in melanoma(PMID: 33734579).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MX2 antibody 13278-1-AP | Download protocol |
| IHC protocol for MX2 antibody 13278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit. | ||
J Virol mTOR Dysregulation by Vaccinia Virus F17 Controls Multiple Processes with Varying Roles in Infection. | ||
J Virol YTHDF2 Is Downregulated in Response to Host Shutoff Induced by DNA Virus Infection and Regulates Interferon-Stimulated Gene Expression | ||
Nat Commun The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses |