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MXD1 Polyclonal antibody

MXD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19547-1-AP

Print datasheet

Synonyms

MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, MXD1, Protein MAD

验证数据展示

  • WB analysis of HepG2 using 19547-1-AP

    WB analysis of HepG2 using 19547-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19547-1-AP (MXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 19547-1-AP

    IP experiment of HepG2 using 19547-1-AP

    IP result of anti-MXD1 (IP:19547-1-AP, 4ug; Detection:19547-1-AP 1:500) with HepG2 cells lysate 3000ug.

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

ChIPSee 1 publications below

产品信息

19547-1-AP targets MXD1 in WB, IP, chIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationschIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAX dimerization protein 1
Synonyms MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, MXD1, Protein MAD
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberNM_002357
Gene Symbol MXD1
Gene ID (NCBI) 4084
RRIDAB_10641995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05195
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MXD1, also named as MAD, is a transcriptional repressor. MAD1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. There're two isoforms of MXD1 protein.

实验方案

Product Specific Protocols
WB protocol for MXD1 antibody 19547-1-APDownload protocol
IP protocol for MXD1 antibody 19547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

J Biol Chem

Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.

Authors - Jianyin Long
View Article