验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
产品信息
25472-1-AP targets MXRA5 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | matrix-remodelling associated 5 |
| 别名 | Adlican |
| 计算分子量 | 312 kDa |
| 观测分子量 | 163 kDa, 312 kDa |
| GenBank蛋白编号 | NM_015419 |
| 基因名称 | MXRA5 |
| Gene ID (NCBI) | 25878 |
| RRID | AB_2918088 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR99 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MXRA5, or adlican, is an adhesion proteoglycan involved in extracellular matrix (ECM) remodeling and cell-cell adhesion (PMID: 22696596). In kidney, MXRA5 has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli (PMID: 27599751). MXRA5 was identified as a novel biomarker in colorectal cancer, mainly located in the cytoplasm of CRC cells (PMID:23420087). Exome sequencing identified MXRA5 as a novel cancer gene mutated in non-small cell lung carcinoma (PMID: 22696596). MXRA5 was detected 163 kDa in human proximal tubular cells (PMID:27599751).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MXRA5 antibody 25472-1-AP | Download protocol |
| IHC protocol for MXRA5 antibody 25472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells
| ||
Am J Cancer Res Single-cell analysis reveals one cancer-associated fibroblasts subtype linked to metastasis in breast cancer: MXRA5 as a potential novel marker for prognosis | ||
J Cancer Identification of an Immune signature assisted prognosis, and immunotherapy prediction for IDH wildtype glioblastoma |
