• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MYB/c-Myb Monoclonal antibody, PBS Only

MYB/c-Myb Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2C4A5

Cat no : 68542-1-PBS

Print datasheet

Synonyms

MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb

验证数据展示

  • WB analysis using 68542-1-Ig (same clone as 68542-1-PBS)

    WB analysis using 68542-1-Ig (same clone as 68542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68542-1-Ig (MYB/c-Myb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68542-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 68542-1-Ig (same clone as 68542-1-PBS)

    FC experiment of Jurkat using 68542-1-Ig (same clone as 68542-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MYB/c-Myb (68542-1-Ig, Clone:2C4A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68542-1-PBS in a different storage buffer formulation.

产品信息

68542-1-PBS targets MYB/c-Myb in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen MYB/c-Myb fusion protein Ag12021 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-myb myeloblastosis viral oncogene homolog (avian)
Synonyms MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb
Calculated Molecular Weight 640 aa, 72 kDa
Observed Molecular Weight40-50 kDa, 72-80 kDa
GenBank Accession NumberBC064955
Gene Symbol c-Myb
Gene ID (NCBI) 4602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10242
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.