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MYB/c-Myb Recombinant antibody, PBS Only

MYB/c-Myb Uni-rAbTM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230179C11

Cat no : 82804-2-PBS

Print datasheet

Synonyms

MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb

验证数据展示

  • WB analysis using 82804-2-RR (same clone as 82804-2-PBS)

    WB analysis using 82804-2-RR (same clone as 82804-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82804-2-RR (MYB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82804-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 82804-2-RR (same clone as 82804-2-PBS)

    FC experiment of MCF-7 using 82804-2-RR (same clone as 82804-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MYB/c-Myb Recombinant antibody (82804-2-RR, Clone:230179C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82804-2-PBS in a different storage buffer formulation.

产品信息

82804-2-PBS targets MYB/c-Myb in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen MYB/c-Myb fusion protein Ag12021 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-myb myeloblastosis viral oncogene homolog (avian)
Synonyms MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb
Calculated Molecular Weight 640 aa, 72 kDa
Observed Molecular Weight40-50 kDa, 72-80 kDa
GenBank Accession NumberBC064955
Gene Symbol c-Myb
Gene ID (NCBI) 4602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10242
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.