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MYBBP1A Monoclonal antibody, PBS Only

MYBBP1A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2D10

Cat no : 67996-1-PBS

Print datasheet

Synonyms

FLJ37886, MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2

验证数据展示

  • WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

    WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

    WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

    IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67996-1-Ig (MYBBP1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYBBP1A antibody included
  • IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYBBP1A Ready-To-Use IHC Kit. MYBBP1A primary antibody included.

产品信息

67996-1-PBS targets MYBBP1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MYBBP1A fusion protein Ag6008 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name MYB binding protein (P160) 1a
Synonyms FLJ37886, MYB binding protein (P160) 1a, Myb binding protein 1A, MYBBP1A, P160, PAP2
Calculated Molecular Weight 149 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC050546
Gene Symbol MYBBP1A
Gene ID (NCBI) 10514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BQG0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].