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MYBPC2 Polyclonal antibody

MYBPC2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25257-1-AP

Print datasheet

Synonyms

MYBPCF, Fast MyBP C

验证数据展示

  • WB analysis using 25257-1-AP

    WB analysis using 25257-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25257-1-AP (MYBPC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 25257-1-AP

    IP experiment of mouse skeletal muscle using 25257-1-AP

    IP result of anti-MYBPC2 (IP:25257-1-AP, 4ug; Detection:25257-1-AP 1:40000) with mouse skeletal muscle tissue lysate 1040 ug.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25257-1-AP targets MYBPC2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MYBPC2 fusion protein Ag19424 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin binding protein C, fast type
Synonyms MYBPCF, Fast MyBP C
Calculated Molecular Weight 1141 aa, 128 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC136389
Gene Symbol MYBPC2
Gene ID (NCBI) 4606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14324
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYBPC2, also known as MYBPCF, fMyBP-C, is a member of the myosin-binding protein C (MYBPC)family. MYBPC is a myosin-associated protein found in the cross-bridge-bearing zone, or C region, of A bands in striated muscle. MYBPC has three paralogs encoded by unique genes, including slow skeletal (MYBPC1), fast skeletal (MYBPC2), and cardiac (MYBPC3). Slow skeletal MyBP-C (sMyBP-C), fast skeletal MyBP-C (fMyBP-C), and cardiac MyBP-C (cMyBP-C) proteins are highly conserved with over 90% homology. They play unique muscle-specific structural and regulatory roles in actomyosin interactions and contractility in striated muscles, including both cardiac and skeletal muscles (PMID: 35832193, 23936073).

实验方案

Product Specific Protocols
WB protocol for MYBPC2 antibody 25257-1-APDownload protocol
IP protocol for MYBPC2 antibody 25257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols