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验证数据展示

  • WB analysis of human heart using 12022-1-AP

    WB analysis of human heart using 12022-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 12022-1-AP

    WB analysis using 12022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12022-1-AP (MYCBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12022-1-AP

    IHC staining of human pancreas cancer using 12022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYCBP antibody included
  • IHC staining of human pancreas cancer using 12022-1-AP

    IHC staining of human pancreas cancer using 12022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYCBP antibody included
  • IHC staining of mouse colon using 12022-1-AP

    IHC staining of mouse colon using 12022-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12022-1-AP (MYCBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYCBP antibody included
  • IF Staining of HepG2 using 12022-1-AP

    IF Staining of HepG2 using 12022-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYCBP antibody (12022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

MYCBP Polyclonal antibody

MYCBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12022-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

c-Myc-binding protein, Associate of Myc 1, AMY-1, AMY1, AMY 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, A549 cells, human kidney tissue, human lung tissue, human placenta tissue, HEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYCBP Ready-To-Use IHC Kit. MYCBP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12022-1-AP targets MYCBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 MYCBP fusion protein Ag2651 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 c-myc binding protein
别名 c-Myc-binding protein, Associate of Myc 1, AMY-1, AMY1, AMY 1
计算分子量 103 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC008686
基因名称 MYCBP
Gene ID (NCBI) 26292
RRIDAB_2148722
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99417
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.

实验方案

Product Specific Protocols
WB protocol for MYCBP antibody 12022-1-APDownload protocol
IHC protocol for MYCBP antibody 12022-1-APDownload protocol
IF protocol for MYCBP antibody 12022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet
View Article
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
View Article
mouseWB

Acta Physiol (Oxf)

A dual role of miR-22 in rhabdomyolysis-induced acute kidney injury.

Authors - S Mathia
View Article
mouseWB

J Cell Mol Med

Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization.

Authors - Yongqi Wang
View Article
humanWB

Oncol Lett

Overexpression of MYC binding protein promotes invasion and migration in gastric cancer.

Authors - Lijie Gong
  • KD Validated
View Article