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MYCBPAP Polyclonal antibody

MYCBPAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12763-1-AP

Print datasheet

Synonyms

AMAP-1, AMAP1, AMAP 1, AMAM-1, AMAM1

验证数据展示

  • WB analysis of human placenta using 12763-1-AP

    WB analysis of human placenta using 12763-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12763-1-AP (MYCBPAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12763-1-AP

    IF Staining of HepG2 using 12763-1-AP

    Immunofluorescent analysis of HepG2 cells, using MYCBPAP antibody 12763-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human pancreas cancer using 12763-1-AP

    IHC staining of human pancreas cancer using 12763-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 12763-1-AP

    IHC staining of human pancreas cancer using 12763-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HUVEC using 12763-1-AP

    IF Staining of HUVEC using 12763-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using MYCBPAP antibody (12763-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12763-1-AP targets MYCBPAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen MYCBPAP fusion protein Ag3419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MYCBP associated protein
Synonyms AMAP-1, AMAP1, AMAP 1, AMAM-1, AMAM1
Calculated Molecular Weight 947 aa, 108 kDa
Observed Molecular Weight 108 kDa
GenBank Accession NumberBC028393
Gene Symbol MYCBPAP
Gene ID (NCBI) 84073
RRIDAB_2235719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TBZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYCBPAP, or MYCBP associated protein, is a protein encoded by the human MYCBPAP gene. It is primarily expressed in the testis and is involved in spermatogenesis, the process of forming sperm cells. The protein may also play a role in synaptic processes, which are essential for neuronal signaling. MYCBPAP is predicted to be an intracellular protein with molecular functions including clathrin binding, phospholipid binding, and protein binding. It is associated with biological processes such as cell differentiation, chemical synaptic transmission, endocytosis, and spermatogenesis.

实验方案

Product Specific Protocols
WB protocol for MYCBPAP antibody 12763-1-APDownload protocol
IHC protocol for MYCBPAP antibody 12763-1-APDownload protocol
IF protocol for MYCBPAP antibody 12763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci China Life Sci

Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice

Authors - Yiling Zhou
View Article