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MYCBPAP Polyclonal antibody

MYCBPAP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12763-1-AP

Print datasheet

Synonyms

AMAP-1, AMAP1, AMAP 1, AMAM-1, AMAM1

验证数据展示

  • WB analysis of human placenta using 12763-1-AP

    WB analysis of human placenta using 12763-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12763-1-AP (MYCBPAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 12763-1-AP

    IF Staining of HepG2 using 12763-1-AP

    Immunofluorescent analysis of HepG2 cells, using MYCBPAP antibody 12763-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human pancreas cancer using 12763-1-AP

    IHC staining of human pancreas cancer using 12763-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 12763-1-AP

    IHC staining of human pancreas cancer using 12763-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12763-1-AP (MYCBPAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HUVEC using 12763-1-AP

    IF Staining of HUVEC using 12763-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using MYCBPAP antibody (12763-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12763-1-AP targets MYCBPAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MYCBPAP fusion protein Ag3419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MYCBP associated protein
Synonyms AMAP-1, AMAP1, AMAP 1, AMAM-1, AMAM1
Calculated Molecular Weight 947 aa, 108 kDa
Observed Molecular Weight 108 kDa
GenBank Accession NumberBC028393
Gene Symbol MYCBPAP
Gene ID (NCBI) 84073
RRIDAB_2235719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TBZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYCBPAP antibody 12763-1-APDownload protocol
IHC protocol for MYCBPAP antibody 12763-1-APDownload protocol
IF protocol for MYCBPAP antibody 12763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols