验证数据展示
经过测试的应用
| Positive WB detected in | NCI-H1299 cells, U266 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14584-1-AP targets L-Myc in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | L-Myc fusion protein Ag6105 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian) |
| 别名 | bHLHe38, LMYC, L-Myc, MYCL, MYCL1, Protein L Myc 1 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC011864 |
| 基因名称 | MYCL1 |
| Gene ID (NCBI) | 4610 |
| RRID | AB_3669190 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12524 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The MYC gene family consists of 3 members, C-MYC, L-MYC, and N-MYC, all of which belong to the superfamily of basic helix-loop-helix leucine zipper (bHLHLZ) DNA binding proteins. L-MYC encodes a nuclear phosphoprotein with 364 amino acids, heterodimerizing with partner protein Max and acts as a transcription factor to control the expression of target genes. L-Myc is involved in many biological processes, including cell proliferation, differentiation, apoptosis and has been found to be amplified and over-expressed in some malignancies. L-Myc amplification has been reported in small-cell lung cancer (PMID: 25528583, 33524794). The observed molecular weight of L-Myc is 68 kDa, which is consistent with the description in the literature (PMID: 8657155).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for L-Myc antibody 14584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |