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验证数据展示

  • IF Staining of U2OS using 82881-1-RR

    IF Staining of U2OS using 82881-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYCL1 antibody (82881-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green).

  • FC experiment of HeLa using 82881-1-RR

    FC experiment of HeLa using 82881-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MYCL1 Recombinant antibody (82881-1-RR, Clone:230104D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MYCL1 Recombinant antibody

MYCL1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82881-1-RR

产品说明书

CloneNo. 230104D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Protein L-Myc, MYCL, LMYC, Class E basic helix-loop-helix protein 38, bHLHe38

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82881-1-RR targets MYCL1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MYCL1 fusion protein Ag30335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian)
别名 Protein L-Myc, MYCL, LMYC, Class E basic helix-loop-helix protein 38, bHLHe38
计算分子量 40 kDa
GenBank蛋白编号BC011864
基因名称 MYCL1
Gene ID (NCBI) 4610
RRIDAB_3670600
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12524
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MYCL1 antibody 82881-1-RRDownload protocol
FC protocol for MYCL1 antibody 82881-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols