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MYD88 Polyclonal antibody

MYD88 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29946-1-AP

Print datasheet

Synonyms

Myd88

验证数据展示

  • WB analysis using 29946-1-AP

    WB analysis using 29946-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29946-1-AP (MYD88 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 29946-1-AP

    IHC staining of mouse liver using 29946-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29946-1-AP (MYD88 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 29946-1-AP

    IHC staining of mouse liver using 29946-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29946-1-AP (MYD88 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNR8383 cells, RAW 264.7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29946-1-AP targets MYD88 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity mouse, rat
Cited Reactivityrat
Immunogen MYD88 fusion protein Ag30662 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myeloid differentiation primary response gene 88
Synonyms Myd88
Calculated Molecular Weight 34 kDa
Observed Molecular Weight29-34 kDa
GenBank Accession NumberNM_010851
Gene Symbol Myd88
Gene ID (NCBI) 17874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22366
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis. MYD88 is detected in bone marrow.

实验方案

Product Specific Protocols
WB protocol for MYD88 antibody 29946-1-APDownload protocol
IHC protocol for MYD88 antibody 29946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int Immunopharmacol

Echinatin alleviates inflammation and pyroptosis in hypoxic-ischemic brain damage by inhibiting TLR4/ NF-κB pathway

Authors - Xiaoyue Tao
View Article