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  • Featured Product
  • KD/KO Validated

MYEF2 Polyclonal antibody

MYEF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16051-1-AP

Print datasheet

Synonyms

MST156, MEF-2, MEF2, MEF 2, KIAA1341

验证数据展示

  • IHC staining of human breast cancer using 16051-1-AP

    IHC staining of human breast cancer using 16051-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16051-1-AP (MYEF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYEF2 antibody included
  • WB analysis using 16051-1-AP

    WB analysis using 16051-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16051-1-AP

    IF Staining of HepG2 using 16051-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYEF2 antibody (16051-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • IP experiment of Jurkat using 16051-1-AP

    IP experiment of Jurkat using 16051-1-AP

    IP result of anti-MYEF2 (IP:16051-1-AP, 4ug; Detection:16051-1-AP 1:2000) with Jurkat cells lysate 2800ug.

  • FC experiment of C6 using 16051-1-AP

    FC experiment of C6 using 16051-1-AP

    1x10^6 C6 cells were intracellularly stained with 0.25 ug MYEF2 Polyclonal antibody (16051-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy MYEF2 Ready-To-Use IHC Kit. MYEF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16051-1-AP targets MYEF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MYEF2 fusion protein Ag9010 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myelin expression factor 2
Synonyms MST156, MEF-2, MEF2, MEF 2, KIAA1341
Calculated Molecular Weight 600 aa, 64 kDa
Observed Molecular Weight61-64 kDa
GenBank Accession NumberBC014533
Gene Symbol MYEF2
Gene ID (NCBI) 50804
RRIDAB_2146869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2K5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYEF2 antibody 16051-1-APDownload protocol
IHC protocol for MYEF2 antibody 16051-1-APDownload protocol
IF protocol for MYEF2 antibody 16051-1-APDownload protocol
IP protocol for MYEF2 antibody 16051-1-APDownload protocol
FC protocol for MYEF2 antibody 16051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB, IHC, CoIP

Cell Rep

PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.

Authors - Yan Wang
View Article
humanWB,IHC

BMC Gastroenterol

Expression and function of myelin expression factor 2 in hepatocellular carcinoma

Authors - Peng Zhang
  • KD Validated
View Article