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MYH1-motor domain Polyclonal antibody

MYH1-motor domain Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat No : 22282-1-AP

Print datasheet

Synonyms

MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x

验证数据展示

  • WB analysis of human skeletal muscle using 22282-1-AP

    WB analysis of human skeletal muscle using 22282-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22282-1-AP

    IHC staining of human skeletal muscle using 22282-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of human skeletal muscle using 22282-1-AP

    IHC staining of human skeletal muscle using 22282-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MYH1 antibody included
  • FC experiment of C2C12 using 22282-1-AP

    FC experiment of C2C12 using 22282-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22282-1-AP targets MYH1-motor domain in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, heavy chain 1, skeletal muscle, adult
Synonyms MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x
Calculated Molecular Weight 223 kDa
Observed Molecular Weight 223 kDa
GenBank Accession NumberNM_005963
Gene Symbol MYH1
Gene ID (NCBI) 4619
RRIDAB_2879059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12882
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

实验方案

Product Specific Protocols
WB protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
IHC protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
FC protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols