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验证数据展示

  • WB analysis of human skeletal muscle using 22282-1-AP

    WB analysis of human skeletal muscle using 22282-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22282-1-AP

    IHC staining of human skeletal muscle using 22282-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of human skeletal muscle using 22282-1-AP

    IHC staining of human skeletal muscle using 22282-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MYH1 antibody included
  • FC experiment of C2C12 using 22282-1-AP

    FC experiment of C2C12 using 22282-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MYH1-motor domain Polyclonal antibody

MYH1-motor domain Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 22282-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22282-1-AP targets MYH1-motor domain in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 1, skeletal muscle, adult
别名 MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x
计算分子量 223 kDa
观测分子量 223 kDa
GenBank蛋白编号NM_005963
基因名称 MYH1
Gene ID (NCBI) 4619
RRIDAB_2879059
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12882
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

实验方案

Product Specific Protocols
WB protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
IHC protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
FC protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols