MYH1 (N-terminal) Polyclonal antibody

MYH1 (N-terminal) Polyclonal Antibody for IHC, IF-P, IP, ELISA
Cat No. 25182-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

IHC, IF-P, IP, ELISA and More (2)

MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25182-1-AP targets MYH1 (N-terminal) in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, pig, chicken
免疫原 MYH1 (N-terminal) fusion protein Ag17129 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, heavy chain 1, skeletal muscle, adult
别名 MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x
计算分子量 1939 aa, 223 kDa
观测分子量 220-230 kDa
GenBank蛋白编号BC114545
基因名称 MYH1
Gene ID (NCBI) 4619
RRIDAB_2879947
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12882
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

实验方案

Product Specific Protocols
IHC protocol for MYH1 (N-terminal) antibody 25182-1-APDownload protocol
IF protocol for MYH1 (N-terminal) antibody 25182-1-APDownload protocol
IP protocol for MYH1 (N-terminal) antibody 25182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Biol Macromol

Eicosapentaenoic acid-mediated activation of PGAM2 regulates skeletal muscle growth and development via the PI3K/AKT pathway

Authors - Chenchen Li
WB,IF

Int J Mol Sci

miRNA-10a-5p Targeting the BCL6 Gene Regulates Proliferation, Differentiation and Apoptosis of Chicken Myoblasts

Authors - Genxi Zhang
mouseWB

J Agric Food Chem

Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.

Authors - Luchu Zhang
WB,IHC

Cells

MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation.

Authors - Genxi Zhang
mouseWB

J Agric Food Chem

Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.

Authors - Peiyuan Li
human,mouseWB

Sci Rep

MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.

Authors - Hirotaka Iwasaki
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