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  • Featured Product
  • KD/KO Validated

MYH9 Monoclonal antibody, PBS Only

MYH9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D9D2

Cat no : 60233-1-PBS

Print datasheet

Synonyms

DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9

验证数据展示

  • WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

    IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

    WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

    WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.

产品信息

60233-1-PBS targets MYH9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen MYH9 fusion protein Ag1612 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 9, non-muscle
Synonyms DFNA17, EPSTS, FTNS, MHA, MYH9, Myosin 9, Myosin heavy chain 9
Calculated Molecular Weight 227 kDa
Observed Molecular Weight 230 kDa, 160 kDa
GenBank Accession NumberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35579
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).