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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

    IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

    WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

    WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 60233-1-Ig (same clone as 60233-1-PBS)

    IHC staining of human skin cancer using 60233-1-Ig (same clone as 60233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 60233-1-Ig (MYH9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYH9 antibody included
  • Featured Product
  • KD/KO Validated

MYH9 Monoclonal antibody, PBS Only

MYH9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60233-1-PBS

Print datasheet

CloneNo. 5D9D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYH9 Ready-To-Use IHC Kit. MYH9 primary antibody included.

产品信息

60233-1-PBS targets MYH9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen MYH9 fusion protein Ag1612 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 9, non-muscle
Synonyms NMMHC II-a, Myosin-9, Myosin heavy chain, non-muscle IIa, Myosin heavy chain 9, Myosin 9
Calculated Molecular Weight 227 kDa
Observed Molecular Weight 230 kDa, 160 kDa
GenBank Accession NumberBC011915
Gene Symbol MYH9
Gene ID (NCBI) 4627
RRIDAB_2881357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35579
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).