验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, human skeletal muscle tissue, mouse bladder tissue, Jurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MYL12A Ready-To-Use IHC Kit. MYL12A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
16287-1-AP targets MYL12A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | MYL12A fusion protein Ag9413 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin, light chain 12A, regulatory, non-sarcomeric |
| 别名 | MLC 2B, MLCB, MRLC3, MYL12A, MYL2B, Myosin RLC, RLC |
| 计算分子量 | 171 aa, 20 kDa |
| 观测分子量 | 19-20 kDa |
| GenBank蛋白编号 | BC016372 |
| 基因名称 | MYL12A |
| Gene ID (NCBI) | 10627 |
| RRID | AB_2878238 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19105 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL12A antibody 16287-1-AP | Download protocol |
| IHC protocol for MYL12A antibody 16287-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem J Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli. | ||
Front Physiol Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction. | ||
Biofizika [Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium]. | ||
EMBO J Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier |
