验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin, light chain 2, regulatory, cardiac, slow |
| 别名 | MLC 2v, MYL2, MLC 2, MLC2, MLC-2 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | NM_000432 |
| 基因名称 | Myosin Light Chain 2 |
| Gene ID (NCBI) | 4633 |
| RRID | AB_2881342 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10916 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| FC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cell Reports Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4. | ||
iScience SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury. | ||
Int J Mol Med Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis. |
