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MYL3 Monoclonal antibody, PBS Only (Capture)

MYL3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E4B2

Cat no : 66286-1-PBS

Print datasheet

Synonyms

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

验证数据展示

  • WB analysis using 66286-1-Ig (same clone as 66286-1-PBS)

    WB analysis using 66286-1-Ig (same clone as 66286-1-PBS)

    Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • WB analysis of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

    WB analysis of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL3 antibody included
  • IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL3 antibody included
  • Cytometric bead array standard curve of MP50642-3

    Cytometric bead array standard curve of MP50642-3

    Cytometric bead array standard curve of MP50642-3, MYL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66286-1-PBS. Detection antibody: 66286-5-PBS. Standard:Ag24535. Range: 0.098-6.25 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy MYL3 Ready-To-Use IHC Kit. MYL3 primary antibody included.

产品信息

66286-1-PBS targets MYL3 as part of a matched antibody pair:

MP50642-3: 66286-1-PBS capture and 66286-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen MYL3 fusion protein Ag24535 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Synonyms Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC009790
Gene Symbol MYL3
Gene ID (NCBI) 4634
RRIDAB_2881669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08590
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)