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  • Featured Product
  • KD/KO Validated

MYL6 Monoclonal antibody, PBS Only

MYL6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G4C9

Cat No : 68142-1-PBS

Print datasheet

Synonyms

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

验证数据展示

  • WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

    WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68142-1-Ig (MYL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL6 antibody included
  • IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL6 antibody included
  • WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

    WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

    WB result of MYL6 antibody (68142-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYL6 transfected A549 cells. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

    IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (68142-1-Ig, Clone: 2G4C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MYL6 Ready-To-Use IHC Kit. MYL6 primary antibody included.

产品信息

68142-1-PBS targets MYL6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen MYL6 fusion protein Ag13091 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 6, alkali, smooth muscle and non-muscle
Synonyms 17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17-25 kDa
GenBank Accession NumberBC006781
Gene Symbol MYL6
Gene ID (NCBI) 4637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60660
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).