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MYL6B Polyclonal antibody

MYL6B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16963-1-AP

Print datasheet

Synonyms

MLC1SA, MYL6B, Myosin light chain 6B

验证数据展示

  • WB analysis using 16963-1-AP

    WB analysis using 16963-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16963-1-AP (MYL6B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 16963-1-AP

    IHC staining of mouse skeletal muscle using 16963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 16963-1-AP

    IHC staining of mouse skeletal muscle using 16963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHuH-7 cells, mouse testis tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16963-1-AP targets MYL6B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MYL6B fusion protein Ag10683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, light chain 6B, alkali, smooth muscle and non-muscle
Synonyms MLC1SA, MYL6B, Myosin light chain 6B
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC012425
Gene Symbol MYL6B
Gene ID (NCBI) 140465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14649
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL6B, also known as MLC1SA, is primarily found in a hexamer consisting of four light chains and two heavy chains. MYL6B is an essential light chain for non-muscle Myosin II (NMII) that is involved in the control of cell adhesion, cell migration and tissue architecture, cargo transport, and endocytosis (PMID: 29439719, 33817240).

实验方案

Product Specific Protocols
WB protocol for MYL6B antibody 16963-1-APDownload protocol
IHC protocol for MYL6B antibody 16963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols