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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 21527-1-AP (same clone as 21527-1-PBS)

    WB analysis using 21527-1-AP (same clone as 21527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK3 antibody (21527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

MYLK3 Polyclonal antibody, PBS Only

MYLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21527-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated

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产品信息

21527-1-PBS targets MYLK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MYLK3 fusion protein Ag16137 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin light chain kinase 3
别名
计算分子量 819 aa, 88 kDa
观测分子量 85-100 kDa
GenBank蛋白编号BC109097
基因名称 MYLK3
Gene ID (NCBI) 91807
RRIDAB_10734432
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ32MK0
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).