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MYLK3 Polyclonal antibody, PBS Only

MYLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21527-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 21527-1-AP (same clone as 21527-1-PBS)

    WB analysis using 21527-1-AP (same clone as 21527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    IHC staining of human colon using 21527-1-AP (same clone as 21527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK3 antibody (21527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    IF Staining of HepG2 using 21527-1-AP (same clone as 21527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21527-1-PBS in a different storage buffer formulation.

产品信息

21527-1-PBS targets MYLK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MYLK3 fusion protein Ag16137 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin light chain kinase 3
Synonyms
Calculated Molecular Weight 819 aa, 88 kDa
Observed Molecular Weight 85-100 kDa
GenBank Accession NumberBC109097
Gene Symbol MYLK3
Gene ID (NCBI) 91807
RRIDAB_10734432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ32MK0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).