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验证数据展示

  • WB analysis using 24309-1-AP

    WB analysis using 24309-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24309-1-AP

    IP experiment of HEK-293 using 24309-1-AP

    IP result of anti-MYLK4 (IP:24309-1-AP, 4ug; Detection:24309-1-AP 1:500) with HEK-293 cells lysate 3600ug.

  • IHC staining of human heart using 24309-1-AP

    IHC staining of human heart using 24309-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 24309-1-AP

    IHC staining of human heart using 24309-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

MYLK4 Polyclonal antibody

MYLK4 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24309-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA and More (1)

别名

MLCK4, MYLK4, SgK085, Sugen kinase 85

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, L02 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24309-1-AP targets MYLK4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 MYLK4 fusion protein Ag19314 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin light chain kinase family, member 4
别名 MLCK4, MYLK4, SgK085, Sugen kinase 85
计算分子量 388 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC132831
基因名称 MYLK4
Gene ID (NCBI) 340156
RRIDAB_2877632
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYLK4(Myosin light chain kinase family member 4) is also named as SGK085 and belongs to the CAMK Ser/Thr protein kinase family. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MYLK4 antibody 24309-1-APDownload protocol
IHC protocol for MYLK4 antibody 24309-1-APDownload protocol
IP protocol for MYLK4 antibody 24309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.

Authors - Mengkai Yang
  • KD Validated
View Article
IF

bioRxiv

GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis

Authors - Francesca Gobbo
View Article