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MYLPF Polyclonal antibody

MYLPF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16052-1-AP

Print datasheet

Synonyms

HUMMLC2B, MLC2B, MRLC2, MYL11, MYLPF

验证数据展示

  • WB analysis of mouse skeletal muscle using 16052-1-AP

    WB analysis of mouse skeletal muscle using 16052-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16052-1-AP (MYLPF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 16052-1-AP

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 16052-1-AP

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16052-1-AP targets MYLPF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MYLPF fusion protein Ag9013 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin light chain, phosphorylatable, fast skeletal muscle
Synonyms HUMMLC2B, MLC2B, MRLC2, MYL11, MYLPF
Calculated Molecular Weight 169 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC012571
Gene Symbol MYLPF
Gene ID (NCBI) 29895
RRIDAB_2148036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYLPF antibody 16052-1-APDownload protocol
IHC protocol for MYLPF antibody 16052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model.

Authors - Ryohei Oya
View Article
mouseWB

Mol Cell Biol

MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD.

Authors - Magdalena A Cichewicz
View Article