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验证数据展示

  • WB analysis of mouse brain using 24565-1-AP

    WB analysis of mouse brain using 24565-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24565-1-AP (MYO10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 24565-1-AP

    IF Staining of SH-SY5Y using 24565-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 24565-1-AP (MYO10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of RAW 264.7 using 24565-1-AP

    FC experiment of RAW 264.7 using 24565-1-AP

    1X10^6 RAW 264.7 cells were stained with 0.2ug MYO10 antibody (24565-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

  • Featured Product
  • KD/KO验证

MYO10 Polyclonal antibody

MYO10 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 24565-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, FC (Intra), ELISA and More (2)

别名

Unconventional myosin-X, Unconventional myosin-10, Unconventional myosin 10, myosin X, KIAA0799

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24565-1-AP targets MYO10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, zebrafish
免疫原 MYO10 fusion protein Ag18882 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin X
别名 Unconventional myosin-X, Unconventional myosin-10, Unconventional myosin 10, myosin X, KIAA0799
计算分子量 2058 aa, 237 kDa
观测分子量 240-250 kDa
GenBank蛋白编号BC137168
基因名称 MYO10
Gene ID (NCBI) 4651
RRIDAB_2879611
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HD67
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO10 antibody 24565-1-APDownload protocol
IF protocol for MYO10 antibody 24565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Adv

MYO10 drives genomic instability and inflammation in cancer.

Authors - Franklin Mayca Pozo
  • KO Validated
View Article
zebrafishIHC

Elife

Retrograde Ret signaling controls sensory pioneer axon outgrowth.

Authors - Adam Tuttle
View Article
mouseIF

PLoS One

NIMA-related kinase 1 (NEK1) regulates meiosis I spindle assembly by altering the balance between α-Adducin and Myosin X.

Authors - Miguel A Brieño-Enríquez
View Article
humanWB

Proteomics Clin Appl

Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction.

Authors - Yueyan Li
View Article
humanWB

Cytoskeleton (Hoboken)

The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division.

Authors - Ivan Ramirez
View Article