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验证数据展示

  • IHC staining of human bladder using 18828-1-AP

    IHC staining of human bladder using 18828-1-AP

    Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYO16 antibody included
  • IF Staining of A431 using 18828-1-AP

    IF Staining of A431 using 18828-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 18828-1-AP (MYO16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MYO16 Polyclonal antibody

MYO16 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 18828-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

NYAP3, Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 3, MYR8, myosin XVI, MYO16B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman bladder tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MYO16 Ready-To-Use IHC Kit. MYO16 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18828-1-AP targets MYO16 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin XVI
别名 NYAP3, Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 3, MYR8, myosin XVI, MYO16B
计算分子量 1858 aa, 206 kDa
观测分子量 123 kDa, 210 kDa, 106 kDa
GenBank蛋白编号BC146791
基因名称 MYO16
Gene ID (NCBI) 23026
RRIDAB_10858634
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6X6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO16, also named as KIAA0865, MYO16B, is actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO16 may be involved in targeting of the catalytic subunit of protein phosphatase 1 during brain development. The antibody can recognize isoform1, isoform2 and isoform3 of MYO16.

实验方案

Product Specific Protocols
IHC protocol for MYO16 antibody 18828-1-APDownload protocol
IF protocol for MYO16 antibody 18828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols