验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue |
Planning an IHC experiment? We recommend our IHCeasy MYO16 Ready-To-Use IHC Kit. MYO16 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
产品信息
25104-1-AP targets MYO16 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MYO16 fusion protein Ag18798 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin XVI |
| 别名 | KIAA0865, MYO16, Myo16b, myosin XVI, MYR8, Unconventional myosin 16 |
| 计算分子量 | 1858 aa, 206 kDa |
| 观测分子量 | 200-240 kDa |
| GenBank蛋白编号 | BC146791 |
| 基因名称 | MYO16 |
| Gene ID (NCBI) | 23026 |
| RRID | AB_2879898 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6X6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYO16, also named as KIAA0865, MYO16B, is actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO16 may be involved in targeting of the catalytic subunit of protein phosphatase 1 during brain development. The antibody can recognize isoform1, isoform2 and isoform3 of MYO16.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYO16 antibody 25104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Neurosci Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization.
| ||
Mol Biol Cell The TRIM9/TRIM67 neuronal interactome reveals novel activators of morphogenesis.
|