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MYO18B Polyclonal antibody

MYO18B Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25691-1-AP

Print datasheet

Synonyms

MYO18B, myosin XVIIIB

验证数据展示

  • IHC staining of mouse heart using 25691-1-AP

    IHC staining of mouse heart using 25691-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25691-1-AP (MYO18B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO18B antibody included
  • IHC staining of mouse heart using 25691-1-AP

    IHC staining of mouse heart using 25691-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25691-1-AP (MYO18B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO18B antibody included
  • IF Staining of U2OS using 25691-1-AP

    IF Staining of U2OS using 25691-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYO18B antibody (25691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy MYO18B Ready-To-Use IHC Kit. MYO18B primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25691-1-AP targets MYO18B in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MYO18B fusion protein Ag22454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin XVIIIB
Synonyms MYO18B, myosin XVIIIB
Calculated Molecular Weight 2566 aa, 285 kDa
Observed Molecular Weight280-290 kDa
GenBank Accession NumberBC150626
Gene Symbol MYO18B
Gene ID (NCBI) 84700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUG5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MYO18B antibody 25691-1-APDownload protocol
IF protocol for MYO18B antibody 25691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols