• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

MYO1A Polyclonal antibody

MYO1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17499-1-AP

Print datasheet

Synonyms

MIHC, DFNA48, Brush border myosin I, BBM-I, BBMI

验证数据展示

  • WB analysis of HepG2 using 17499-1-AP

    WB analysis of HepG2 using 17499-1-AP

    WB result of MYO1A antibody (17499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1A transfected HepG2 cells.

  • WB analysis of mouse brain using 17499-1-AP

    WB analysis of mouse brain using 17499-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17499-1-AP

    IP experiment of HeLa using 17499-1-AP

    IP result of anti-MYO1A (IP:17499-1-AP, 4ug; Detection:17499-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IHC staining of human colon cancer using 17499-1-AP

    IHC staining of human colon cancer using 17499-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17499-1-AP (MYO1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1A antibody included
  • IF Staining of HepG2 using 17499-1-AP

    IF Staining of HepG2 using 17499-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYO1A antibody (17499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, HeLa cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MYO1A Ready-To-Use IHC Kit. MYO1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17499-1-AP targets MYO1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen MYO1A fusion protein Ag11682 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin IA
Synonyms MIHC, DFNA48, Brush border myosin I, BBM-I, BBMI
Calculated Molecular Weight 1043 aa, 118 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC059387
Gene Symbol MYO1A
Gene ID (NCBI) 4640
RRIDAB_2148172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO1A antibody 17499-1-APDownload protocol
IHC protocol for MYO1A antibody 17499-1-APDownload protocol
IF protocol for MYO1A antibody 17499-1-APDownload protocol
IP protocol for MYO1A antibody 17499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell Rep Med

Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer

Authors - Yi Zhao
View Article